Journal: European Journal of Microbiology & Immunology

Article Title: Evidence for the presence of Borrelia burgdorferi in invasive breast cancer tissues

doi: 10.1556/1886.2024.00021

Figure Lengend Snippet: Representative images of CXCL8 and CXCL10 inflammatory markers on B. burgdorferi- positive and negative breast cancer and normal breast tissues. Panels: B and C show B. burgdorferi -positive breast cancer tissues stained Borrelia monoclonal antibody (green) and CXCL8 monoclonal antibody (red). Panels: F and G show B. burgdorferi -positive breast cancer tissues stained Borrelia monoclonal antibody (green) and CXCL10 monoclonal antibody (red). Panels: D and H show no staining on B. burgdorferi -negative breast cancer tissue samples for both inflammatory markers, CXCL8 and CXCL10 respectively. Panels: A and E show negative control; normal breast tissues are negative for B. burgdorferi and both inflammatory markers. White scale bar: 100 µm

Article Snippet: Different sets of tissue array slides were stained for CXCL8 using polyclonal IL8/CXCL8 rabbit antibody (Cat#A2541, ABclonal Technology, Woburn, MA, USA) and for CXCL10 using polyclonal CXCL10 antibody (Cat#DF6417, Affinity Biosciences, Cincinnati, OH, USA).

Techniques: Staining, Negative Control

Journal: European Journal of Microbiology & Immunology

Article Title: Evidence for the presence of Borrelia burgdorferi in invasive breast cancer tissues

doi: 10.1556/1886.2024.00021

Figure Lengend Snippet: A summary diagram of the B. burgdorferi , CXCL8 and CXCL10-positive and negative breast cancer tissues with a total of 70 cases

Article Snippet: Different sets of tissue array slides were stained for CXCL8 using polyclonal IL8/CXCL8 rabbit antibody (Cat#A2541, ABclonal Technology, Woburn, MA, USA) and for CXCL10 using polyclonal CXCL10 antibody (Cat#DF6417, Affinity Biosciences, Cincinnati, OH, USA).

Techniques:

Journal: Cell Death & Disease

Article Title: Tumor cell-derived EMP1 is essential for cancer-associated fibroblast infiltration in tumor microenvironment of triple-negative breast cancer

doi: 10.1038/s41419-025-07464-9

Figure Lengend Snippet: A , B A mixture of TNBC cells and CAFs was injected subcutaneously into the mammary gland of nude mice, and the morphology and weight of the xenograft tumors formed by TNBC cells are shown in the plots. C Tumor volume growth curve of xenograft tumors in the CAF+ sh-NC_TNBC cell line (sh-NC) and the CAF+ sh-EMP1_TNBC cell line (sh-EMP1) groups. D The body weight of mice in the CAF+ sh-NC_TNBC cell line (sh-NC) group and the CAF+ sh-EMP1_TNBC cell line (sh-EMP1) group. E The expression of CAF biomarkers in xenograft tumors was determined by western blotting assay. F The CAF infiltration level in xenograft tumors is examined by IHC, Masson, and Sirius Red staining assays. G Knockdown of EMP1 in TNBC cells significantly inhibited CAF infiltration in xenograft tumors. Multicolor immunofluorescence experiments were performed in the xenograft tumors between sh-NC + CAF group and sh-EMP1 + CAF group. The CAFs are labeled by fibroblast biomarker anti-α-SMA with green fluorescence. The EMP1 expression in xenograft tumors was evaluated using anti-EMP1 bodies with red fluorescence. ** P < 0.01.

Article Snippet: Primary EMP1 antibodies (dilution 1:200, Cat# ab230445, Abcam, Cambridge, UK) and α-SMA antibodies (1:400, Cat# 27095-1-AP, Proteintech, Wuhan, China) were used to evaluate the EMP1 and α-SMA protein level in TNBC tissues.

Techniques: Injection, Expressing, Western Blot, Staining, Knockdown, Immunofluorescence, Labeling, Biomarker Assay, Fluorescence